Journal: Journal of Pain Research

Article Title: Oxycodone regulates incision-induced activation of neurotrophic factors and receptors in an acute post-surgery pain rat model

doi: 10.2147/JPR.S180396

Figure Lengend Snippet: The altered expression of neurotrophic receptors in the spinal cord 24 hours after PI and the effects of oxycodone on neurotrophic receptors. Notes: ( A , C , and E ) RT-qPCR was performed to examine the mRNA level of TrkA, TrkB, and TrkC. ( B , D , and F ) The upper panels represent results of the Western blots. The lower panels show the semi-quantitative analysis of TrkA, TrkB, and TrkC after normalization to the corresponding β-actin. (* P <0.05, ** P <0.01 vs control group; # P <0.05, ## P <0.01, ### P <0.001 vs PI, one-way ANOVA followed by Bonferroni’s multiple comparison post hoc test, n=3–4 rats per group). Each value represents mean±SEM. PI+OXY, postoperative oxycodone group; OXY+PI, preoperative oxycodone group. Abbreviations: PI, plantar incision; RT-qPCR, real-time quantitative PCR; SEM, standard error of the mean; Trks, tyrosine kinase receptors.

Article Snippet: Membranes were first blocked with 5% (w/v) defatted milk in 0.1% Tween 20 (TBST; 2 mmol/L Tris–HCl, 50 mmol/L NaCl, pH 7.4) for 2 hours at room temperature and followed by incubation overnight at 4°C with specific primary antibody for OPRM1 (1:1,000, A7264; ABclonal, Wuhan, China), NGF (1:1,000, ab52918; Abcam, Cambridge, UK), BDNF (1:500, DF6387; Affinity, Whhan, China), NT-3 (1:1,000, DF6105; Affinity), TrkA (1:200, BA0404; Boster, Wuhan, China), TrkB (1:200; Affinity), and TrkC (1:500, A14033; ABclonal, Wuhan, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control, Comparison, Real-time Polymerase Chain Reaction